VolRC RAS scientific journal (online edition)

Journal section "Fodder production, feeding of farm animals, and fodder technology"

Comparing Feed Samples by the Content of Essential Amino Acids

Artamonov I.V.

Volume 5, Issue 1, 2022

Artamonov I.V. (2022). Comparing feed samples by the content of essential amino acids.Agricultural and Livestock Technology, 5 (1). DOI: 10.15838/alt.2022.5.1.1 URL: http://azt-journal.ru/article/29175?_lang=en

DOI: 10.15838/alt.2022.5.1.1

Abstract   |   Authors   |   References
The article presents the results of amino acid analysis of various types of feed for cattle. We chose methionine (in the form of methionine-sulfone), lysine, leucine, isoleucine, histidine, and valine as marker amino acids. The analysis was performed by high-performance liquid chromatography using Na-ion exchange columns with post-column derivatization. Samples of silage, grass mixtures, compound feed obtained from various producers and livestock farms were studied. Also, in the course of the work, we revealed the limitations of the combination of equipment and methodology used, including the lower limit of the concentration of amino acids in the analyzed samples. The purpose of the study was to determine the differences in the amino acid composition of different types of feed. The tasks of the study were as follows: approbation of the method for determining the amino acid composition in the laboratory, analysis of the spectrum of feed samples, comparison of their quantitative amino acid content. This approach, consisting in the analysis of diets from the point of view of elementary composition, is relatively new. At the same time, there exist scientifically substantiated opportunities for balancing diets both through changing the composition of feed mixtures, taking into account their amino acid composition, and through the introduction of individual amino acids or additives with a high content of amino acids into feed mixtures


protein, feed, High-performance liquid chromatography, ion exchange chromatography, amino acids